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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310641702
中文名称：
英文名称：Comb, 2.0mm, 16 samples
货期：热销品现货，其他请咨询
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文献和实验The ribonuclease protection assay (RPA)
. Place the samples in a heat block pre-warmed to 90°C. Immediately turn the temperature to 56°C (allowing the temperature to ramp down slowly) and incubate for 12-16 hours. Turn the heat block to 37°C for 15 minutes prior to the RNase treatments
(optional; see Steps 21 and 37) Horizontal gel electrophoresis tank for agarose gels (for duplex PCR only; see Steps 40-53) Standard DNA sequencing gel apparatus with 31 x 38.5-cm glass plates, 0.4-mm spacers, and a shark's-tooth comb (for hot-stop PCR
Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
as follows:MDE gel 16.0mlddH2O 44.2ml10X TBE 3.84ml10% APS 256µlTEMED 25.6µlPour sequencing gel format with appropriate sharkstooth comb. Gel will polymerize in about 1 hour.Loading Buffer95% formamide10mM NaOH0.025% Bromophenol Blue0.025% Xylene CyanolRun
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