赛默飞世尔Thermo Fisher X125 0,2ML

Polyclonal and Monoclonal Antibodies

1) After collecting, blood should be allowed to clot for 60 min . at 37 C or O.N at 4 C. 2) Separate the clot from the sides of the tube (ringing) using a pasteur pipette. Place clot at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate

Stripping Western Blots

by tapping the tube (try to avoid getting beads stuck to the side of the tube, above the level of the reaction mixture). 14.) Incubate at 25oC (room temp.) for 25min, mixing occasionally. 15.) Add sample buffer to 1x (I usually add 20 μl of 3x

原代神经细胞培养方法 Neuron Cell Culture

: number of cells counted x25/9x10,000x2mls e.g. 125 cells x 25/9 x 10,000 x 2mls = 6,944,444.5 cells in 2mls