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999
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北京泽平
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货号:T_70311436397
中文名称:
英文名称:Tube rack s/steel J2-19 test tube for 19mm tubes
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文献和实验ES CELL DNA EXTRACTION: TUBE ME
in a sealed humid container at 60℃ for 1 hour. (Meanwhile, label eppy tubes!) 3. Transfer the contents of each well into a separate 1.5ml eppendorf tube (µ-cent. tube). 4. Continue lysing the cells for 2 more hours. (60℃). (Float tubes
ES CELL DNA EXTRACTION: TUBE ME
, with vacuum flask). Do not disturb the pellet. Vacuum droplets from walls of tube as well. 17. Half fill the tube with 70% EtOH and re-spin for 2 minutes at 4℃. 18. Suck off supernatant as in step 16. 19. Store the open tube on the bench at TRm
ES CELL DNA EXTRACTION: TUBE METHOD
of each well into a separate 1.5ml eppendorf tube (µ-cent. tube). 4. Continue lysing the cells for 2 more hours. (60°ree;C). (Float tubes in waterbath- don't need tupperware). 5. Add an equal volume (250 µl) of phenol:chloroform (phenol: chloroform: isoamylalcohol, 25
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