赛默飞世尔Thermo Fisher X12 FILTER

原创：western protocol

1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture

Southern Hybridization Protocols

(Polyvinyl pyrrolidone) (Fisher Bioreagents) 300mg BSA (A-9647 Sigma) Top up autoclaved H2O up to 30ml (do NOT autoclave solution: BSA will coagulate!) Filter with 0.2 m . Store at -20℃ Preparation of 50% dextran sulphate (10ml) 5g Dextran sulphate 10ml