赛默飞世尔Thermo Fisher X1000 CAPIL

Mouse Compete Blood Counts

of the total.   Red cell count: Dilute cells 1/1000 in PBS. Add 10 µL to a hemocytometer. Count the number of RBC per large square. Calculate: RBC/large square x 1,000 dilution x 10 large squares/µL = RBC/µL blood.   White cell count: Add 10 µL

SDS-PAGE

) protein, load only 0.0125 OD ml. For Drosophila tissue culture cells, suspend 3X106 cells per 90 µl cracking buffer, and load about 20 µl per lane (0.8 mm thick gel with the BRL gel box and 20 well comb). 10. Running. Use a constant Amps power supply

RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes

probe       10x Taq Buffer 2.5 µl   DIG DNA Labeling Mix, 10 x conc. 2.5 µl   Primer * (10 pmol/ul) 1.0 µl   Purified PCR product (at least 200 µg/ml) 4.0 µl   Taq Polymerase 2 units