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货号:T_70103-339-15
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英文名称:SPIN VANE F/3-5ML VIALS 6/CS
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文献和实验bacteriophage and plasmid DNA. Nature468(7320):67–71. doi: 10.1038/nature09523 19. Cong L, Ran FA, Cox D, Lin S, Barretto R,Habib N, Hsu PD, Wu X, Jiang W, Marraffi niLA, Zhang F (2013) Multiplex genome engineeringusing CRISPR/Cas systems. Science339(6121
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
Single and Double FISH protocols for Drosophila
1. Chlorine bleach; diluted 1:1 with water. 2. 40% formaldehyde solution (prepared fresh from paraformaldehyde as described below). 3. 10X PBS solution. 4. Heptane. 5. Methanol. 6. 20 ml Disposable Glass Scintillation Vials (Fisher).
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