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文献和实验Delta-delta Ct method and PCR efficiencies-Real-Time PCR
. If one can't get good efficiencies with a broad-range standard curve, will it accurately measure expression in tissues with low expression? This is a key point for me because I expect my GOI to be very tissue specific. I did alread optimize for primer
Southern Blot Analysis(from Baker lab, university of Florida
of Yeastchromosomal DNA per lane to be run. Digest 0.05 to 0.01μg of plasmid DNA(a massive overkill).2.Allow restriction digest to proceed at 37°C overnight. Electrophoresis 1.Seal the gel casting platform at both ends with tape. Prepare and pour a100mL
Southern Blot Analysis(from Baker lab, university of Florida)
of plasmid DNA(a massive overkill).2.Allow restriction digest to proceed at 37℃ overnight.Electrophoresis1.Seal the gel casting platform at both ends with tape.Prepare and pour a100ml 0.8% agarose in 1X TBE.Insert the comb making sure there are no airbubbles
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