赛默飞世尔Thermo Fisher 容量瓶 FLASK V

Cool growth competent E . coli

2O; add 10 ml 250 mM KCl; pH to 7.0 with NaOH Adjust vol to 1 liter; Autoclave. Just before use, add 5 ml sterile 2M MgCl2 per liter TB per liter 10 mM PIPES Na salt 3.35 g 15 mM CaCl2 (Fisher) 2.2 g 250 mM KCl (Aldrich) 18.64g 55

重组DNA的分离、克隆与测序实验

1. Sambrook, J., Fritsch, E.F., and Maniatis, T., in Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, NY, Vol. 1, 2, 3 (1989). 2. Glover, D.M. DNA Cloning Volume I: A Practical Approach. IRL Press, Oxford, 1985.  

重组DNA的分离、克隆与测序实验手册

contained in a 1.5 ml microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20℃ overnight at this stage.2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15 minutes