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文献和实验Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos Cheng Cui1 , Brenda Rongish1 , Charles Little1 , and Rusty Lansford2 ,3 1 Department of Anatomy and Cell Biology, University of Kansas Medical Center
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Syringe (1-mL) (Fisher Scientific) Thermo plate (MATS) METHOD Experiment Set-Up 1. Prepare culture media (two dishes of cleavage medium with 10% SPS, and one dish of 2 mM DMAP): i. Place 10-15 drops
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
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