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英文名称:Power cordCABLE-4with output connectors 55V 50Hz f, 1/Cs, MA: FB57059
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文献和实验V294.PART II,Chapter 5 细胞粘附实验(英文版)
PART II:BASIC CELL MIGRATION AND RELATED ASSAYS vol.294 Guan J.-L. (ed.) Cell Migration-Developmental Methods and Protocols Chapter 5 Cell-Adhesion Assays Dennis F. Kucik and Chuanyue Wu Summary One of the most important
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
). We typically cut with 100 pulses at 2.5 kHz and power set to about 0.27mW (average power measured at specimen with Coherent FieldMaxII power meter and laser set to continuous at 2500 Hz). The ablation laser power setting will be consistent for cutting axons
RNA purification using TRizol--TRIZOL法提取RNA
microcentrifuge tubes.b.Centrifuge for 1 minute to pellet the cells.c.Pour off the supernatant.d.Add 1 ml of TRIzol or TRI reagent to the tubes.e.Lyse cells by repetitive pipetting.f.Centrifuge homogenate at 12000 x g for 10 minutes at 4℃.g.Transfer the homogenate
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