赛默飞世尔Thermo Fisher X960 Pipett

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis

Loss of Heterozygosity

microdissected cells in 20 µl proteinase K buffer.Incubate overnight at 37°C.B: Prepare the Glass PlatesTIP: Use Accuwipes for cleaning purposes, as they will not leave lint behind and are non-abrasive.Clean glass plates twice with glass cleaner.Repeat using 95

Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period

Placing the tube on tissue paper for few seconds downward. Be careful from cross-contamination between different samples. Add 400 µl of nucleic lysis buffer to eppendorf tube. Note: if the pellet formed, you must pipette the pellet up to dissolve