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货号:T_70310565912
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英文名称:DISPENSER FOR 150X150MM CLOSE-IT mm
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文献和实验Small scale DNA preps for Neurospora crassa.
to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S-23) plus 500 microliters of isolation buffer (50 mM Tris-HCl pH 8, 170 mM EDTA pH 8, 1 N-lauroylsarcosine) to each tube and vortex
Small scale DNA preps for Neurospora crassa.
, or until the cultures are saturated. 2. Spin the culture tubes for five minutes to compress the mycelia, then invert and blot the tubes on a paper towel to remove most of the supernatant. 3. Add approximately 150 microliters of Ottawa Sand (Fisher Scientific # S
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
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