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999
- 供应商：
北京泽平
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货号：T_701AA45065TC
中文名称：
英文名称：M1L 400 WIRE 0.25MM DIA 227G
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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
7.5), 1 mM EDTA, 10 mM MgCl 2 , 10 µM GTP, 10 µg/ml GDP antibody, and 2 nM fluorescent nucleotide tracer; for the protocol below, this will be prepared as a 1.1× solution (18 µl added to a final 20 µl
mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
A safe method of extracting DNA from Coccidioides immitis
in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth
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