赛默飞世尔Thermo Fisher Unbelegte,

Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)

(Fisher Scientific) Chemotaxis Buffer    25 mM Hepes, pH 7.4 1% (v/w) Endotoxin-free Bovine Serum Albumin prepare in RPMI 1640 medium

In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes

or into 6 mm x 50 mm culture tubes). 5) Using a 10 mg/ml stock of Proteinase K (PrK), make solutions of 25 ug/ml and 40 ug/ml PrK in PBTw. Divide the gonads in half. Digest one half of the gonads in 3 ml of 25 ug/ml PrK / PBTw for 30 min and the other half

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

after each use.    4)      Prepare myocardial construct media by adding 10% horse serum, 2% fetal bovine serum, 1% penicillin-streptomycin, and 6 mg/mL  -aminocaproic acid into DMEM. 50 μg/ml of ascorbic acid and 2 μg/ml of insulin in 25 μM HEPES