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文献和实验H-Thymidine Uptake by Cultured Cells
cap bottle inside a double light-tight box. Dip the slide in the emulsion and drain momentarily. Place the slides vertically on a test tube rack in an oven set at 28° C for at least 1 hour in darkness.
H-Thymidine Uptake by Cultured Cells
-3 emulsion is stored refrigerated as a gel in a screw cap bottle inside a double light-tight box. 10.Dip the slide in the emulsion and drain momentarily. Place the slides vertically on a test tube rack in an oven set at 28° C for at least 1 hour in darkness
). A combination of T4 DNA polymerase and Klenow DNA polymerase are used to "fill-in" the DNA fragments by catalyzing the 3'-5' incorporation of complementary nucleotides into resultant double-stranded fragments with a 5' overhang. Additionally, the single-stranded
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