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文献和实验We have been karyotyping animals in the Grainger lab to confirm that the stocks maintained in the lab are indeed X. tropicalis and to establish that wild-caught animals being used for gynogenesis are also X. tropicalis . We recently
E-Z 96 X-press Plasmid Vacuum Protocol
well plate inside the manifold base; 3) Place the top plate of manifold over the base, the deep well plate or waste collection tray now should be positioned under the E-Z 96 X-press Plate. Seal the unused wells of E-Z 96 Xpress Plate with sealing
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
sec. break x 3 times, 20% amplitude with a small tip). Now the length of your chromatin DNA should be 200-1000 bp. (13) Centrifuge for 20 min at 14000 rpm, 4°C. Recover supernatant. This is the soluble sheared chromatin fraction. This can be stored
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