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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
- 规格:
EA
货号:T_70119-818-975
中文名称:
英文名称:XYLENE TUBES 10/BX
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Heat at 94°C for 10 min. Precipitate the DNA using 0.1 x volume 3 M sodium acetate, and 2.5 x volume 100% ethanol. Resuspend the DNA pellet in 40 µl water. To 12.2 µl DNA from #10 above, add the following re-amplification
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