sartorius赛多利斯16522Glass/polypr

SDS-PAGE Western Blotting Protocols

glass plate can be treated with Rain-X to facilitate later removal of the gel. 2. Slide into holder without tightening the screws. 3. Place on pouring stand, making sure that the glass plates are pushed all the way to the bottom before tightening

Acid-adjusted Swine Serum

ml bottles) for 15 min. 4. Pull off serum by aspiration (peelet is soft and will not stick to bottle). 5. Filter serum successively through 1.2 µm, .85µm and .65 µm filters using 142 mm filter holder. 6. Adjust pH to 7.0 using 1N NaOH. 7. Heat

RLGS protocol

with a sonicator for 5 min. Cover the top of the gel with Kim Towel wetted by 1 x TBE containing 1% glycerin to keep wet. 　 G. in situ digestion After electrophoresis remove the cathodal top buffer with aspirator and take out each gel holder. Expel the gel