Sartorius赛多利斯13400-150------KG

Purification of recombinant sBRF M166L

and/or protein assay.Typical recovery is 2 to 6 mg BRF protein. -------------------------------------------------------------------------------- Lysis/Binding buffer,pH 8.0 (150 ml) 30mM Tris 8.0 0.54 g Tris 300mM NaCl2.63 g NaCl 6.0M Gu-HCl86 g Guanidine

体外荧光法检测核内体早期动力学

O and can be stored in aliquots at −20 °C. PBS The solution contains 150-mM NaCl and 20-mM Na2 HPO4 (disodium hydrogen phosphate), pH 7.4 (with HCl). It can be stored at room temperature (i.e., at 21 °C

Yeast Chromatin Immunoprecipitation (ChIP)

protocol -------------------------------------------------------------------------------- ChIP Buffers 2.5 M glycine (filter sterilize,store at room temperature) 1x TBS+ 125 mM glycine (store at 4 degrees C) 1x TBS (store at 4 degrees C) ChIP lysis