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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Lanosta-8,20(22)-dien-26-oic acid, 15-hydroxy-3,11,23-trioxo-, (15α,20Z)-
- 供应商:
上海汇谱仪器有限公司
- CAS号:
1961358-01-9
- 规格:
5mg
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
Phosphoamino acid analysis:Mark Kamps method
. If you are using another rig, you will have to optimize your electrophoresis times. 11. Dry plate in oven. 12. Spray plate with ninhydrin sol'n for several seconds. Incubate in oven until can see purple spots of PAA standards. 15 min should be plenty
120 3.80 3.23 2.89 2.67 2.51 2.39 2.30 2.22 2.16 2.05 1.94 1.82 1.69 1.53 1.31 120 240 3.75 3.17 2.84 2.62 2.46 2.34 2.24 2.17 2.10 2.00 1.89 1.77 1.63 1.46 1.20 240 ∞ 3.69 3.12 2.79 2.57 2.41 2.29 2.19 2.11 2.05 1.94 1.83 1.71 1.57 1.39 1.00
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