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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
500μg/10μg/1mg/50μg
| 规格： | 500μg | 产品价格： | ￥17480.0 |
|---|---|---|---|
| 规格： | 10μg | 产品价格： | ￥1380.0 |
| 规格： | 1mg | 产品价格： | ￥25680.0 |
| 规格： | 50μg | 产品价格： | ￥3680.0 |
产品信息
| 别名 | Sonic Hedgehog Protein; SHH; HHG-1; SHH |
| 物种 | Mouse |
| 表达宿主 | E.coli |
| 序列信息 | Cys25-Gly198 |
| 检索号 | Q62226 |
| 分子量 | 19.8 kDa |
| 表观分子量 | 20 kDa |
产品特性
| 纯度 | >95% as determined by reducing SDS-PAGE. |
| 内毒素 | < 1.0 EU per µg as determined by LAL test. |
| 保存 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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文献和实验with a Ca2+ -free medium or with trypsin/dispase. The latter facilitates the introduction of recombinant or trappable probes and microimaging studies of, for example, changes in cytosolic-free Ca2+ concentration or the dynamics of individual organelles
Purification of Recombinant Poly(ADP-Ribose) Polymerases
, more than 11 mg of pure and highly active mouse PARP-2 can be obtained from 1 L of Sf9 insect cell culture.
PRIMARY MOUSE KERATINOCYTE CULTURES Isolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol . 254:3-20 (1995). The epidermis from a newborn mouse should yield
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