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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Rat IgG2b,κ
- 保存条件:
2 - 8℃避光,切勿冻存
- 克隆性:
IM7
- 标记物:
FITC
- 级别:
流式抗体
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 应用范围:
流式检测
- 浓度:
5 μL /Test
- 靶点:
CD44, MIC4, CSPG8
- 规格:
100T(F1104401-100)/25T(F1104401-25)
| 规格: | 100T(F1104401-100) | 产品价格: | ¥677.6 |
|---|---|---|---|
| 规格: | 25T(F1104401-25) | 产品价格: | ¥308.0 |
| 商品名 | Anti-Human/Mouse CD44, FITC(Clone:IM7)流式抗体 |
|---|---|
| 靶点 | CD44, MIC4, CSPG8 |
| 标记 | FITC |
| 仪器 | 适用488nm激光器的流式细胞仪 |
| 亚型 | Rat IgG2b, κ |
| 克隆号 | IM7 |
| 推荐应用 | 流式检测 |
| 用量 | 5 μL /Test |
| 纯化 | 亲和层析 |
| 储存液 | 含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2) |
| 保存条件 | 2 - 8℃避光,切勿冻存 |
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Protocol for intracytoplasmic staining of cytokines for FACS analysis
+ 1% BSA, + 5% heat inactivated normal rabbit serum (HINRS). 9) Label with antibodies to surface molecules that are resistant to fixation (30 mins 4 deg C) eg. anti-CD4 (H129.19-Quantum Red; Sigma R 3637) for FL3 anti-CD44 (Pgp-1:IM7
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