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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
7-Ethyl-10-hydroxycamptothecin
- 供应商:
上海汇谱仪器有限公司
- CAS号:
86639-52-3
- 规格:
25mg
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文献和实验Phycoerythrin conjugation protocol
(Molecular Probes) DTT Dithiothreitol NEM N-ethyl maleimide * 10 ml Sephadex G-10 columns (2) Separation column A long Sephacryl S-300 column
Quantitation of Acetaldehyde-DNA Adducts: Biomarkers of Alcohol Consumption
step-by-step information on the analysis of N 2 -ethyl-dGuo in DNA by LC-ESI-MS/MS-SRM. The protocol described easily can be applied to DNA isolated from different tissues and cell types and on DNA amounts as low as 5–10 μg.
chloroform-isoamyl alcohol (v/v, 24:1). 5. precipitate the DNA with 3-M ammonium acetate and 2.5 volume of 100% ethyl alcohol. 6. wash the DNA pellet with 70% ethanol 7. dry the pellete in speed vacuum centrifuge for 10 minutes.
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