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- 规格：
100管/96样/50管/48样
| 规格： | 100管/96样 | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 50管/48样 | 产品价格： | ￥820.0 |
肌酸激酶(CK)测试盒
（1）肌酸显色法（比色法）
磷酸肌酸+ADP
肌酸 + ATP
肌酸+α-萘酚
红色化合物(λ=540nm)
在一定范围内，红色深浅与肌酸量成正比，从而可求得血清中CK活性。在反应体系中加入Mg作激活剂；半胱氨酸供给巯基，保持CK活性中心必需基团不被氧化；和硫酸锌沉淀蛋白并中止反应。
（2）酶偶联法
磷酸肌酸+ADP
检测意义
(2) 酶偶联法：男24～195U/L，女24～170U/L。
增高：
(1) 主要用心肌梗死诊断，CK升高幅度较AST和LDH都大，且出现早，2-4小时开始升高，12-48小时达到高峰，2-4天恢复到正常。尤其对心肌缺血和心内膜下心肌梗死的诊断比其他酶灵敏度高。故动态检测CK变化有助于观察病情和预后估计。
(2) 还见于进行性肌营养不良发作期、病毒性心肌炎、多发性心肌炎、肌肉损伤或手术后、脑血管疾病、酒精中毒、甲状腺功能减退、肺梗塞等。
减低：见于甲状腺功能亢进症。
注意事项
(1) 肌酐、精氨酸、胍乙酸等也可与α-萘酚及产生颜色反应。故肾功能衰竭患者应用自身血清做空白对照，以消除肌酐的影响。
(2) α-萘酚为白色或略带黄色之结晶，颜色过深，应在乙醇中重结晶后再用。
(3) Mg为激活剂，半胱氨酸提供巯基，和硫酸锌沉淀蛋白并中和反应。
(4) 血清CK活性>200U/L时，应用已知CK活性正常的血清稀释后重测，其结果乘以稀释倍数。
(1) 最好使用血清作标本，也可用肝素抗凝血浆，因CK活性不稳定，室温仅能稳定4h，4℃仅稳定8～12h，故标本采集后应尽快分离血清，及时测定。不能及时测定，应避光、低温保存。温度升高引起的酶失活为不可逆的。
(2) 红细胞内不含CK，轻度溶血无影响，但中、重度溶血可因红细胞内释放出的AK、ATP及G6P，使CK值假性升高。
(3) Mg、Ca、Mn对该酶有激活作用，Cl、So、Po、枸橼酸、氟化物可抑制其活性。
肌酸+ATP
ATP+葡萄糖
ADP+6-磷酸葡萄糖
6-磷酸葡萄糖+NADP
6-磷酸葡萄糖+NADPH
利用酶偶联反应原理连续监测NADP还原生成NADPH，后者引起340nm吸光度的增高。在340nm监测单位时间内NADPH的生成量(ΔA/min)，可计算出CK的活性浓度。
式中6220为NADPH在340nm的摩尔吸光度，2.30为反应液的总体积(ml)，0.10为血清用量(ml)。ΔA/min为平均每分钟的吸光度变化值。
（2）肌酸显色法（比色法）
检测样本
人类血清
样本要求
1. 空腹采血，采血前不宜做剧烈运动。
2. 可用干燥管、肝素抗凝管采血加塞送检。
3. 避免采用溶血、乳穈状样品。血液分离血清后2～8℃可稳定一周。
肌酸激酶(CK)测试盒
Creatine kinase (CK) test kit
(1) Creatine colorimetric method (colorimetric method)
Phosphate creatine+ADP
Creatine+ATP
Creatine+diacetyl+ α- Naphthol
Red compound（ λ= 540nm)
Within a certain range, the depth of red color is directly proportional to the amount of creatine, thus determining the CK activity in serum. Add Mg as an activator in the reaction system; Cysteine supplies thiol groups to maintain the essential functional groups of CK active centers from oxidation; Barium hydroxide and zinc sulfate precipitate the protein and terminate the reaction.
(2) Enzyme coupling method
Phosphate creatine+ADP
Creatine+ATP
ATP+glucose
ADP+6-phosphoglucose
Glucose 6-phosphate+NADP
Glucose 6-phosphate+NADPH
Continuous monitoring of NADP reduction to NADPH using the principle of enzyme coupling reaction, which causes an increase in absorbance at 340nm. Monitoring the generation of NADPH per unit time at 340nm（ Δ A/min, the active concentration of CK can be calculated.
2. Calculation method
(1) Enzyme coupling method
In the formula, 6220 is the molar absorbance of NADPH at 340nm, 2.30 is the total volume of the reaction solution (ml), and 0.10 is the serum dosage (ml). Δ A/min is the average absorbance change value per minute.
(2) Creatine colorimetric method (colorimetric method
Testing samples
Human serum
Sample requirements
1. For fasting blood collection, it is not advisable to engage in vigorous exercise before blood collection.
2. Dry tubes and heparin anticoagulant tubes can be used to collect blood and plug it for testing.
3. Avoid using hemolytic or milky samples. Blood can remain stable for one week at 2-8 ℃ after serum separation.
Creatine kinase (CK) test kit
Detection significance
1. Normal reference value
(1) Creatine colorimetric method: 8-60U/L (0.5-3.6U).
(2) Enzyme coupling method: Male 24-195U/L, female 24-170U/L.
2. Clinical significance of creatine kinase
Height increase:
(1) Mainly used for diagnosis of myocardial infarction, the increase in CK is greater than that of AST and LDH, and it appears earlier. It starts to rise at 2-4 hours, reaches its peak at 12-48 hours, and returns to normal after 2-4 days. Especially for the diagnosis of myocardial ischemia and subendocardial myocardial infarction, the sensitivity is higher than other enzymes. Therefore, dynamic detection of CK changes can help orve the condition and estimate prognosis.
(2) It can also be seen during the onset of progressive muscular dystrophy, viral myocarditis, multiple myocarditis, muscle injury or surgery, cerebrovascular disease, alcoholism, hypothyroidism, pulmonary infarction, etc.
Reduced: seen in hyperthyroidism.
matters needing attention
1. Creatine colorimetric method
(1) Creatinine, arginine, guanidinoacetic acid, etc. can also be combined with α- Naphthol and diacetyl produce color reactions. Therefore, patients with renal failure should use their own serum as a blank control to eliminate the influence of creatinine.
(2) α- Naphthol is a white or slightly yellow crystal with a dark color. It should be recrystallized in ethanol before use.
(3) Mg serves as an activator, cysteine provides thiol groups, barium hydroxide and zinc sulfate precipitate proteins and neutralize the reaction.
(4) When the serum CK activity is greater than 200U/L, the serum with known normal CK activity should be diluted and retested, and the result should be multiplied by the dilution factor.
2. Enzyme coupling method
(1) It is best to use serum as a sample, or heparin anticoagulant plasma can be used. Due to the unstable activity of CK, it can only be stable at room temperature for 4 hours and at 4 ℃ for 8-12 hours. Therefore, serum should be separated as soon as possible after sample collection and measured in a timely manner. Unable to measure in a timely manner, should be kept away from light and stored at low temperatures. The enzyme inactivation caused by temperature increase is irreversible.
(2) Red blood cells do not contain CK, mild hemolysis has no effect, but moderate to severe hemolysis can falsely increase CK values due to the release of AK, ATP, and G6P from red blood cells.
(3) Mg, Ca, and Mn have an activating effect on the enzyme, while Cl, So, Po, citric acid, and fluoride can inhibit its activity.
（1）肌酸显色法（比色法）
磷酸肌酸+ADP
肌酸 + ATP
肌酸+α-萘酚
红色化合物(λ=540nm)
在一定范围内，红色深浅与肌酸量成正比，从而可求得血清中CK活性。在反应体系中加入Mg作激活剂；半胱氨酸供给巯基，保持CK活性中心必需基团不被氧化；和硫酸锌沉淀蛋白并中止反应。
（2）酶偶联法
磷酸肌酸+ADP
检测意义
1．正常参考值
(1) 肌酸显色法：8～60U/L(0.5～3.6U)。(2) 酶偶联法：男24～195U/L，女24～170U/L。
2. 肌酸激酶临床意义
增高：
(1) 主要用心肌梗死诊断，CK升高幅度较AST和LDH都大，且出现早，2-4小时开始升高，12-48小时达到高峰，2-4天恢复到正常。尤其对心肌缺血和心内膜下心肌梗死的诊断比其他酶灵敏度高。故动态检测CK变化有助于观察病情和预后估计。
(2) 还见于进行性肌营养不良发作期、病毒性心肌炎、多发性心肌炎、肌肉损伤或手术后、脑血管疾病、酒精中毒、甲状腺功能减退、肺梗塞等。
减低：见于甲状腺功能亢进症。
注意事项
1. 肌酸显色法
(1) 肌酐、精氨酸、胍乙酸等也可与α-萘酚及产生颜色反应。故肾功能衰竭患者应用自身血清做空白对照，以消除肌酐的影响。
(2) α-萘酚为白色或略带黄色之结晶，颜色过深，应在乙醇中重结晶后再用。
(3) Mg为激活剂，半胱氨酸提供巯基，和硫酸锌沉淀蛋白并中和反应。
(4) 血清CK活性>200U/L时，应用已知CK活性正常的血清稀释后重测，其结果乘以稀释倍数。
2. 酶偶联法
(1) 最好使用血清作标本，也可用肝素抗凝血浆，因CK活性不稳定，室温仅能稳定4h，4℃仅稳定8～12h，故标本采集后应尽快分离血清，及时测定。不能及时测定，应避光、低温保存。温度升高引起的酶失活为不可逆的。
(2) 红细胞内不含CK，轻度溶血无影响，但中、重度溶血可因红细胞内释放出的AK、ATP及G6P，使CK值假性升高。
(3) Mg、Ca、Mn对该酶有激活作用，Cl、So、Po、枸橼酸、氟化物可抑制其活性。
肌酸+ATP
ATP+葡萄糖
ADP+6-磷酸葡萄糖
6-磷酸葡萄糖+NADP
6-磷酸葡萄糖+NADPH
利用酶偶联反应原理连续监测NADP还原生成NADPH，后者引起340nm吸光度的增高。在340nm监测单位时间内NADPH的生成量(ΔA/min)，可计算出CK的活性浓度。
2. 计算方法
（1）酶偶联法式中6220为NADPH在340nm的摩尔吸光度，2.30为反应液的总体积(ml)，0.10为血清用量(ml)。ΔA/min为平均每分钟的吸光度变化值。
（2）肌酸显色法（比色法）
检测样本
人类血清
样本要求
1. 空腹采血，采血前不宜做剧烈运动。
2. 可用干燥管、肝素抗凝管采血加塞送检。
3. 避免采用溶血、乳穈状样品。血液分离血清后2～8℃可稳定一周。
肌酸激酶(CK)测试盒
Creatine kinase (CK) test kit
(1) Creatine colorimetric method (colorimetric method)
Phosphate creatine+ADP
Creatine+ATP
Creatine+diacetyl+ α- Naphthol
Red compound（ λ= 540nm)
Within a certain range, the depth of red color is directly proportional to the amount of creatine, thus determining the CK activity in serum. Add Mg as an activator in the reaction system; Cysteine supplies thiol groups to maintain the essential functional groups of CK active centers from oxidation; Barium hydroxide and zinc sulfate precipitate the protein and terminate the reaction.
(2) Enzyme coupling method
Phosphate creatine+ADP
Creatine+ATP
ATP+glucose
ADP+6-phosphoglucose
Glucose 6-phosphate+NADP
Glucose 6-phosphate+NADPH
Continuous monitoring of NADP reduction to NADPH using the principle of enzyme coupling reaction, which causes an increase in absorbance at 340nm. Monitoring the generation of NADPH per unit time at 340nm（ Δ A/min, the active concentration of CK can be calculated.
2. Calculation method
(1) Enzyme coupling method
In the formula, 6220 is the molar absorbance of NADPH at 340nm, 2.30 is the total volume of the reaction solution (ml), and 0.10 is the serum dosage (ml). Δ A/min is the average absorbance change value per minute.
(2) Creatine colorimetric method (colorimetric method
Testing samples
Human serum
Sample requirements
1. For fasting blood collection, it is not advisable to engage in vigorous exercise before blood collection.
2. Dry tubes and heparin anticoagulant tubes can be used to collect blood and plug it for testing.
3. Avoid using hemolytic or milky samples. Blood can remain stable for one week at 2-8 ℃ after serum separation.
Creatine kinase (CK) test kit
Detection significance
1. Normal reference value
(1) Creatine colorimetric method: 8-60U/L (0.5-3.6U).
(2) Enzyme coupling method: Male 24-195U/L, female 24-170U/L.
2. Clinical significance of creatine kinase
Height increase:
(1) Mainly used for diagnosis of myocardial infarction, the increase in CK is greater than that of AST and LDH, and it appears earlier. It starts to rise at 2-4 hours, reaches its peak at 12-48 hours, and returns to normal after 2-4 days. Especially for the diagnosis of myocardial ischemia and subendocardial myocardial infarction, the sensitivity is higher than other enzymes. Therefore, dynamic detection of CK changes can help orve the condition and estimate prognosis.
(2) It can also be seen during the onset of progressive muscular dystrophy, viral myocarditis, multiple myocarditis, muscle injury or surgery, cerebrovascular disease, alcoholism, hypothyroidism, pulmonary infarction, etc.
Reduced: seen in hyperthyroidism.
matters needing attention
1. Creatine colorimetric method
(1) Creatinine, arginine, guanidinoacetic acid, etc. can also be combined with α- Naphthol and diacetyl produce color reactions. Therefore, patients with renal failure should use their own serum as a blank control to eliminate the influence of creatinine.
(2) α- Naphthol is a white or slightly yellow crystal with a dark color. It should be recrystallized in ethanol before use.
(3) Mg serves as an activator, cysteine provides thiol groups, barium hydroxide and zinc sulfate precipitate proteins and neutralize the reaction.
(4) When the serum CK activity is greater than 200U/L, the serum with known normal CK activity should be diluted and retested, and the result should be multiplied by the dilution factor.
2. Enzyme coupling method
(1) It is best to use serum as a sample, or heparin anticoagulant plasma can be used. Due to the unstable activity of CK, it can only be stable at room temperature for 4 hours and at 4 ℃ for 8-12 hours. Therefore, serum should be separated as soon as possible after sample collection and measured in a timely manner. Unable to measure in a timely manner, should be kept away from light and stored at low temperatures. The enzyme inactivation caused by temperature increase is irreversible.
(2) Red blood cells do not contain CK, mild hemolysis has no effect, but moderate to severe hemolysis can falsely increase CK values due to the release of AK, ATP, and G6P from red blood cells.
(3) Mg, Ca, and Mn have an activating effect on the enzyme, while Cl, So, Po, citric acid, and fluoride can inhibit its activity.
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文献和实验相关实验
大鼠 肌酸激酶 (CK) 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中 肌酸激酶(CK) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 大鼠肌酸激酶 (CK) 水平。用纯化的 大鼠肌酸激酶 (CK) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 肌酸激酶 (CK) ，再与 HRP 标记的 肌酸激酶
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠肌酸激酶 (CK)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 CK 单抗包被于酶标板上，标准品和样品中的 CK 与单抗结合，加入生物素化的抗大鼠 CK ，形成免疫复合物连接在板上，辣根
CK-MB可以比CK高, 国内及国外的很多报道有上述现象,只要CKMB的升高不是由于溶血造成的,CKMB >CK,这样的病例95%是O型或B型血的癌症患者,原因是部分癌症病人免疫系统混乱，其中的一些免疫球蛋白充当的辅酶的作用。 在临床检测中，有时会出现CKMB检测结果比CK还要高的情况，会给临床医生造成一定的困扰。首先介绍免疫抑制法检测CK-MB活性的原理：应用单克隆抗体与CK中的M 亚基结合进而抑制肌酸激酶同工酶CK-MM 以及CK-MB中的M 亚基的活性后,再通
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肌酸激酶(CK)测试盒
￥820 - 1500
