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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 规格：
500μg/10μg/1mg/50μg
| 规格： | 500μg | 产品价格： | ￥11880.0 |
|---|---|---|---|
| 规格： | 10μg | 产品价格： | ￥1780.0 |
| 规格： | 1mg | 产品价格： | ￥17280.0 |
| 规格： | 50μg | 产品价格： | ￥4980.0 |
产品信息
| 别名 | Catabolin, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endoge_x0002_nous Mediator (LEM), Mononuclear Cell Factor (MCF) |
| 物种 | Mouse |
| 表达宿主 | E.coli |
| 序列信息 | MVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS with polyhistidine tag at the C-terminus. |
| 检索号 | P10749.1 |
| 分子量 | 18.3 kDa |
| 表观分子量 | 17 kDa |
| 标签 | His-tag at the C-terminus |
| 生物活性 | Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is <8 pg/mL. The specific activity of recombinant mouse IL-1 beta is approximately >1.2x 10^8 IU/mg. |
产品特性
| 纯度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 内毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution,store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 运输 | Ambient temperature or ice pack. |
| 制剂 | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4. |
| 复融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介绍
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文献和实验参考文献
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Formononetin Inhibits Microglial Inflammatory Response and Contributes to Spinal Cord Injury Repair by Targeting the EGFR/MAPK Pathway(2023/03/28)
作者：Haiping Fu, Mingdong Li, Yanqiang Huan
期刊：IMMUNOLOGICAL INVESTIGATIONS
DOI：10.1080/08820139.2023.2183135
影响因子 ：2.800
Mouse Strains and Genetic Nomenclature
., and Montagutelli, X. 2009. Genetic analysis of skull shape variation and morphological integration in the mouse using interspecific recombinant congenic strains between C57BL/6 and mice of the Mus spretus species. Evolution 63:2668‐2686
V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
, the DCC has been used to study neurite outgrowth(12). We have previously used the DCC to elucidate the role of Rho family proteinsin the chemotactic response of BAC1.2F5 macrophages to CSF-1 (13). Wedescribe the use of this chamber to study the response
起，再在其后标明杂交的代数（如F1、F2等）。 对品系或种群的名称可使用通用的缩写名称。 例如：（C57BL/6 DBA/2）F1 = B6D2F1 （ NMRI X LAC ）F2 3 实验动物的繁殖方法 由于近交系与封闭群动物具有不同的遗传特点，因此应分别选择相应的繁殖方法。 3.1 近交系动物的繁殖方法 选择近交系动物繁殖方法的原则是保持近交系动物的同基因性及其基因纯合性。 3.1.1 引种 作为繁殖用原种的近交系动物必须遗传背景明确
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