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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse IgG1,κ
- 保存条件:
2 - 8℃避光,切勿冻存
- 克隆性:
40131
- 标记物:
PE
- 级别:
流式抗体
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 应用范围:
流式检测
- 浓度:
5 μL /Test
- 靶点:
CD127, IL7R, IL7RA
- 规格:
100T(F11127A02-100)/25T(F11127A02-25)
| 规格: | 100T(F11127A02-100) | 产品价格: | ¥1724.8 |
|---|---|---|---|
| 规格: | 25T(F11127A02-25) | 产品价格: | ¥812.0 |
| 商品名 | Anti-Human CD127, PE (Clone: 40131) 检测试剂 |
|---|---|
| 靶点 | CD127, IL7R, IL7RA |
| 标记 | PE |
| 仪器 | 适用488nm, 532nm, 561nm激光器的流式细胞仪 |
| 亚型 | Mouse IgG1, κ |
| 克隆号 | 40131 |
| 推荐应用 | 流式检测 |
| 用量 | 5 μL /Test |
| 纯化 | 亲和层析 |
| 储存液 | 含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2) |
| 保存条件 | 2 - 8℃避光,切勿冻存 |
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文献和实验性T细胞。如图2显示,CD4细胞经分选被为分CD127-高表达,CD25中等-偏弱表达(CD127hiCD25int-lo)和CD127弱表达CD25高-中等表达(CD127loCD25hi-int)两个细胞群体。所有的调节性T细胞均存在于CD127loCD25hi-int群体内。经分选的CD127loCD25hi-int细胞群体阳性表达FoxP3,而CD127hiCD25int-lo群体则完全不表达FoxP3。从人类外周血单个核细胞中分选有活性T调节方法简述如下:通过BD IMag™ Human CD
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
from whole blood. The Foxp3 Whole Blood Staining Kit includes the PE-conjugated PCH101 clone for human Foxp3 and staining buffers specifically designed for fixation and permeabilization of cells in whole blood, resulting in robust detection of human Foxp
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