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- 2025年07月15日
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文献和实验Calmodulin as an Affinity Purification Tag
Calmodulin is a small (148 amino acids, 17 kDa) ubiquitous protein in eukaryotes, and is considered the primary intracellular calcium sensor, making it a key regulator of intracellular signal transduction. Upon calcium binding, calmodulin
than the succinimidyl esters of the same acids. The “tag” denoted in the figure represents the group added to the protein and may take many forms. View Image
Amino Acid Measurement in Body Fluids Using PITC Derivatives
with phenylisothiocyanate (PITC) in the presence of a coupling solvent. The phenylthiocyanate (PTC) derivatives are then applied to a reverse-phase (RP) HPLC column and separated using a gradient elution system. The PTC amino acids are detected using an ultraviolet (UV
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