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文献和实验Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
and order or configuration of these genes in the MHC. Finally, methods to assess the degree of polymorphism in C4A and C4B proteins and whether low protein levels of plasma C4 may be caused by low C4 gene dosages and/or by mutant C4 genes.
the column with 3 l of Buffer D using a fast flow rate. 2. Elute the column with a 4 l gradient from 100 mM KCl to 500 mM KCl in Buffer D. Collect 25 ml fractions (400 drops/fraction). Actin-containing fractions are identified by dot blot using mAb C4
Immunoaffinity Methods for Purification of Complement Components and Regulators
“polishing” steps, such as gel filtration to remove aggregates. Elution of the bound protein from the antibody-solid phase is commonly achieved by subjecting the column to extremes of pH to disrupt antibody-antigen interaction. Some complement proteins
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