sartorius赛多利斯16407--25----ALK聚

Pheromone Halo Assay

  -Use sterile technique and sterile solutions throughout this method.- 1. Grow a starter culture at 30 C with shaking (250 rpm) until it reaches saturation. *** It is important to make sure all strains to be tested are completely

Transformation of E. coli by Electroporation

for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250

超级感受态细胞的制备

1 for details) 55 mM MnCl2 ・4H2 O 15 mM CaCl2 ・2H2 O 250 mM KCl 10 mM PIPES (0.5M, pH 6.7) Chilled to 0ºC before use. Nucleic Acids And Oligonucleotides Plasmid DNA (recombinant plasmid