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文献和实验Inhibition of Glycogen Synthase Kinase-3 Methods in Molecular Biology v468. chapter 5 Abstract There are two homologous forms of glycogen synthase kinase (GSK)-3, GSK-3a and GSK-3b, whichplay overlapping roles in the regulation of Wnt
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
) DMEM/F12 (Invitrogen, Catalog # 12500-096 or equivalent) Materials: BG01V human embryonic stem cells Tissue culture dishes (60 mm; Fisher, Catalog # 08-772B, 100 mm; Fisher, Catalog # 08-772E, or equivalent) 15 mL conical
Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and V Domain Antibodies
This protocol describes a method for creation of a highly diverse and functional synthetic phage-displayed repertoire of fully human domain antibodies (dAbs). The repertoire is based on two human frameworks (one VH and one Vκ) that express
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