- ￥300
- Sartorius
- 13806--47----ACN
- 德国
- 2025年11月05日
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- 文献和实验
- 技术资料
- 库存：
大量
- 保修期：
一年
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现货
- 供应商：
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- 规格：
盒
sartorius赛多利斯13806--47----ACNCN green - dark green, sterile, 0.45 µm
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文献和实验Green lab protocol for vacuum infiltration transformation of Arabidopsis
sufficient. 10. For each plant sterilize up to 100 µl of seeds (approximately 2500 seeds) and plate out individually. Sterilize seeds (7 minutes rocking in 50% bleach/0.02% Triton X-100, 3 rinses in sterile distilled water). Resuspend seeds
Raft culture media (aka Green’s media)
Add the following to the FCS and sterile filter before adding to the media; 400m l of Adenine (45mM) 100m l of Insulin (5mg/ml) 40m l of Hydrocortisone (1mg/ml) 1ml of Transferrin (500m g/ml) 13.5m l of T3 (1m
Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
) and allow it to stand at room temperature fo 30-45 minutes (Sol. A) 3) For each transfection, dilute 50 µg GFP DNA in 100µl of OptiMem® (Life Technologies) serum-free medium in a sterile microfuge tube (Solution B). 4) Combine Solutions A and B
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