sartorius赛多利斯13006--47----ACRC

Construction and Characterization of Adenovirus Vectors

stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide

DNA Methylation Analysis by Bisulfite Sequencing (BS)

buffer 1.5 µL of 50 mM MgCl2 5 µL of 2 mM dNTPs 0.125 µL of each BS oligonucleotide (100 µM) 0.2 µL of FastStart Taq DNA Polymerase (comment 4) Sterile water up to a final volume of 46 µL

Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos

) and allow it to stand at room temperature fo 30-45 minutes (Sol. A) 3) For each transfection, dilute 50 µg GFP DNA in 100µl of OptiMem® (Life Technologies) serum-free medium in a sterile microfuge tube (Solution B). 4) Combine Solutions A and B