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北京泽平
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EA
货号:T_70311748569
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英文名称:X4 AMMONIA NEUTRALIZATION SOLUTION
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文献和实验Preparation and Staining of Frozen Tissue Sections
-freezing matrix. Block endogenous peroxidase activity by incubating the slides in 0.3% H2 O2 solution in PBS for 10 minutes. Rinse slides 3x in PBS, 2 minutes each time. Block non-specific binding by incubating with blocking buffer (10
Clone Genes From a Phage Library
nylon filters from Fisher (MSI N00HY08250, 82 mm, 50/box). These are quite cheap. Solutions: LB, SM solution, Church hybridization solution, Church wash solution, Chloroform (see the end for recipes).Ex-assist phage as well for plasmid excision
for several weeks.Use 50 microliters per plate. Filters: I like simple uncharged nylon filters from Fisher (MSI N00HY08250, 82 mm, 50/box). These are quite cheap. Solutions: LB, SM solution, Church hybridization solution, Church wash solution, Chloroform
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