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- T_70102-671-57
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70102-671-57
中文名称：
英文名称：COVER GLASS 25X60MM .5MM THICK
货期：热销品现货，其他请咨询
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文献和实验Immunostaining of Paraffin Sections
, in 400 ml of 50 µg/ml polylysine (Sigma #P1524; make up in 10 mM Tris, pH 8) for 30 min. Cover with foil and let dry overnight at room temperature. Polylysine solution can be stored at -20°C and reused. Store coated slides at 4°C and use
should be clean each time. Outside the hood, prepare 5 glass staining dishes for the next steps (75 x 25 mm, for 16 slides, holds slides vertically). Approximately 55 � 60 ml of the solutions are enough to cover the slides. Immediately after removal from 80
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
tubes (6 mm ID x 50 mm). Washes are done by low-speed spins and all solutions (except fixatives) contain 0.1% Tween 20 to prevent gonads from sticking to glass. The key to making this work consistently is to fix dissected gonads in a combination
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