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文献和实验Procedures for In Vitro Production of Bovine Embryos
-Plates (08-758-2)Hyclone1-800-HYCLONEM199 w/o phenol red and w/ Hank’s salts (for oocyte collection medium) SH3A1529.01ICN Biochemicals 1-800-854-05307X Cleaning solution (76-670-95)Invitrogen Tissue Culture Medium 199 with Earle’s salts, for Oocyte
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
upon the amount of tissue used. 10. Count the cells using a hemacytometer and add the appropriate number to a nontreated polystyrene dish containing NEP complete medium. Cell density should be 1 x 106 cells per 6-cm plate, or 2 x 106 cells per 10-cm
conical centrifuge tubes (BD Biosciences, cat. no. 352097) Centrifuge 25‐cm2 (T‐25) tissue culture flasks (Thermo Scientific
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