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货号:T_701ICN2672002
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英文名称:PLANTCON PLANT TISSUE C PK
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora
LIPID EXTRACTION GENERAL METHODOLOGIES
even in some solvents !!Any tissue which cannot be extracted immediately should be frozen as rapidly as possible in dry ice or better in liquid nitrogen and stored in sealed glass containers at -70C. Fresh or frozen tissues should be homogenized in the chosen solvent
A safe method of extracting DNA from Coccidioides immitis
(Identi-plug, Fisher). When there has been sufficient growth, the whole container is transferred to an autoclave and steamed at 1 atm (100 C) for 15 min. This raises the temperature of the culture to about 95 C (as measured in trials with a thermocouple
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