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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent tubes from collapsing. 8. Pass supernatant through 50 micron Miracloth (Calbiochem #475855, La Jolla, CA) in a funnel into fresh Sarstedt tube. 9. Measure
, at 20,000 RPM (k=402), the time required to sediment a particle of100 S, in water at 20 ° C is calculated by: t = 402/100or 4.02 hours (4 hrs, 1 min) Table F.2 presents the k values for the Sorvall SS-34 rotor and for the Sorvall GSA rotor
), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
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