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文献和实验Preparation and Staining of Frozen Tissue Sections
Preparation and Staining of Frozen Tissue Sections for use with Pharmingen reagents I. Preparation of Frozen Sections for Sectioning Materials neeed: 2-methylbutane (isopentane) Liquid Nitrogen Dry ice
In Situ HYBRIDIZATION TO TISSUE SECTIONS
as described for paraffin sections. Embed unfixed embryos or larvae immediately in O.C.T. Place a drop of O.C.T. directly on the chuck. Immerse the chuck in liquid nitrogen but do not let the nitrogen cover the top or touch the O.C.T
Polyclonal and Monoclonal Antibodies
medium. 3) Resuspend in 1 ml of ice cold freezing solution. 4) Transfer vial to an insulated freezing box and place at -70 C for at least 1 hr. (could be for a couple of days). 5) Transfer the vial from the -70 C to the liquid nitrogen
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