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999
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北京泽平
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货号：T_70312131660
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英文名称：ANGLE ROTOR S150AT
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent tubes from collapsing. 8. Pass supernatant through 50 micron Miracloth (Calbiochem #475855, La Jolla, CA) in a funnel into fresh Sarstedt tube. 9. Measure
A quick RNA mini-prep for Neurospora mycelial cultures
. This should take about 5 minutes. 6. Add 1.1 ml of 3M KCl, invert to mix, keep on ice for 10 min. Solution should form semi-solid, flocculent mass as K-SDS precipitate forms. 7. Spin at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent
to an empty BioRad spin column. Add 1.5 ml TE. Place column in 13 x 100 mm glass test tube. Spin in clinical centrifuge (12-place fixed angle rotor) on setting 2 for ~1 min until all liquid is out of the resin (don't over centrifuge as this may damage the resin
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