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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
) at temperature appropriate for used restriction enzyme; Add 0.5ml Tris-buffer-saturated phenol-chroroform, vortex; Centrifuge with Eppendorf 5414 or equivalent at 10000 x g for 2 minutes; Transfer the upper aqueous phase to clean 1.5ml
RNA purification using TRizol--TRIZOL法提取RNA
Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol
Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
that is very common in detritus in ponds and in the solid portion of sewage. The heavily granulated cytoplasm makes a very nice model for observing cytoplasmic streaming. Find the specimen using dark field mode at 40x. Chaos are very large, so this shouldn't
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