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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70111-389-122
中文名称：
英文名称：GLOVE BOX COMPACT SIZE C
货期：热销品现货，其他请咨询
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文献和实验Metallomics Using Inductively Coupled Plasma Mass Spectrometry
and sealing them in the glove chamber with a rubber stopper (see protocol 6 , step 2) or septum before removal from the glove box and use. It is best to let permeable items, particularly septa, sit in the glove box at least overnight to allow absorbed oxygen
Large-scale yeast transformation
, add 40 mls YPAD, grow 2-3 hours more. Spin down 100 ul of the culture (=1 transformation's worth) and check pellet size. Should be approx 3 ul. If much greater, use less culture for following steps 2 . Spin down cells. 2000 r.p.m
Large-scale yeast transformation
for 15-30 mins, on side. Heat shock at 45o C for 15 mins. Pellet cells at 3000 r.p.m. for 15 mins at least. Yeast form diffuse layer on bottom of well. Tip PEG off quickly and carefully. Wipe PEG off top of box. Put caps on. Resuspend
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