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- 文献和实验
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- 库存：
999
- 供应商：
北京泽平
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- 保修期：
1年
- 规格：
EA
货号：T_70315188334
中文名称：
英文名称：SHAKER INCUBATOR W/COOLING COIL
货期：热销品现货，其他请咨询
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
in 37°C shaker incubator (usually 6-8h) Add 4.5 ml of a sterile glycerol-media solution (5ml of autoclaved 80% glycerol solution + 4ml LB + 9µl 1000x tetracyclin stock). Mix well. Aliquot into sterile centrifuge tubes (1ml/tube
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
with the tissue pieces and the collagenase into a tube rack on an orbital shaker inside a 37° C incubator or oven. Turn the orbital shaker on at approximately 60 rpm and close the door. Set a timer for 7 minutes. Be sure to place the collagenase
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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