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文献和实验Determination of Molecular Size by Zonal Sedimentation Analysis on Sucrose Density Gradients
Ultracentrifuge (Beckman LS‐70M or equivalent) Alternate Protocol 1: Zonal Sedimentation Using Sucrose Gradients Formed by a Gradient
Large-scale yeast transformation大规模转化酵母【Yale University】
10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. 2.I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100) 3.Agar
Procedures for In Vitro Production of Bovine Embryos
and oocytes. From left to right are 1) a 1 cc syringe with an extension of rubber tubing connected to a Unopette , 2) a wiretrol (from Drummond Scientific; we purchase from Fisher ), 3) the same device as #1 without the rubber tubing extension and 4) a 5
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