赛默飞世尔Thermo Fisher PHOTODOCIT

V294.PART II，Chapter 2 Boyden法体外趋化试验

of ethylene diamine tetraacetic acid (0.53 mM) and 0.01 g of phenolred in 1 L of phosphate-buffered saline, pH 7.4. 7. 2.5% (w/v) Trypsin (cat. no.15090-046; Gibco Invitrogen): dilute 1:25 in Versenebefore use. 8. Collagen from calf skin (cat. no. C

V294.PART II，Chapter 5 细胞粘附实验（英文版）

; denatured at 85°C for 10 minprior to use). 5. α Minimum essential medium (α-MEM; Life Technologies). 6. Substrate solution: 3.75 mM p-nitropheno-N-acetyl-β-D-glucosaminide (Sigma)in 50 mM citrate buffer, pH 5.0 (aliquoted and stored at –20°C

v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测

Electrophoresis (PAGE) for APC Truncated Forms 1. Laemmli Sample buffer (4×): 250 mM Tris-HCl, pH6.8, 8%(w/v) sodium dodecyl sulfate (SDS), 40% (w/v) glycerol,and 0.1% (w/v) bromophenol blue. Store at room temperatureand add β-mercaptoethanol to 560 mM