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- 技术资料
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999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70110-273-18
中文名称：
英文名称：BENCHTOP DRY ICE FREEZE DRYER
货期：热销品现货，其他请咨询
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
onto Whatman #44 filter paper. Wrap flat mycelial pads in aluminum foil and freeze in dry ice. Do not freeze in EtOH/dry ice bath because alcohol might seep through foil. Pads can be stored at -70°C for at least three weeks. 3. Wash a mortar and pestle
The ribonuclease protection assay (RPA)
in a microfuge for 15 minutes at 4°C. 6. Carefully remove the supernatant and add 100 µl of ice cold 90% ethanol to the pellet. Spin in a microfuge for 5 minutes at 4°C. 7. Carefully remove the supernatant and air dry the pellets
, the supernanant contains mostly nuclei with one or two small phase dark dots attached. The identity of these dots has not been determined. The nuclei can be pelleted by centrifugation at 6,000 rpm for 12 min in SS34 rotor. Freeze on dry ice.
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