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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70189-101-316
中文名称:
英文名称:RET SPLIT FISH PROBE (V 2) 1 S
货期:热销品现货,其他请咨询




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文献和实验Basic Fluorescent in situ Hybridization (FISH)
实验概要 Fluorescence in situ hybridization method is a kind of physical map drawing method, use fluorescent element mark probe, to detect probe and split the chromosome or split between the middle period of the chromatin hybrid
.1 RNA Probe Preparation (see Note 1 ) 1. 1.5 mL microcentrifuge tubes or standard 96-well V-bottom microplates. 2. RNAse free water. 3. T7, T3 or SP6 RNA Polymerase (Fermentas Life Sciences, Burlington, ON, Canada
) was from Fisher and triethylamine (TEA, Cat. No. T-0886) was from Sigma. Fluorophores were purchased from Amersham (Cy3, Cat. No. PA23001; Cy3.5, Cat. No. PA23501; Cy5, Cat. No. PA25001) and Molecular Probes (Oregon Green 488, Cat. No. O-6147; Alexa Fluor 488
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