赛默飞世尔Thermo Fisher CENTRIVAP 1
2-17 MM ACID-RESISTANT ROTOR Röhrchen 44x 12-13mm u. 24x 16-17mmDm. 货号：T_70310484412- ￥25000 - 500000
- Thermo Fisher
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- 2025年07月10日
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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310484412
中文名称：
英文名称：CENTRIVAP 12-17 MM ACID-RESISTANT ROTOR Röhrchen 44x 12-13mm u. 24x 16-17mmDm.
货期：热销品现货，其他请咨询
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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
as little acetic acid with it as possible from the tube and place on a positively charged slide (ex. Superfrost Plus from Fisher). 8. Blot away excess acetic acid if necessary. 9. Place a large coverslip on the slide and press down firmly
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