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999
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北京泽平
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货号:T_701MIR6106
中文名称:
英文名称:TRANSIT-INSECT REAGENT 10X1ML
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文献和实验Pulse-Chase mRNA decay assay in HeLa Tet-off cells:
about 50 ml for 48 wells -12 experiments of 4 time-points). Plate 1 ml per well in 12-well plates. Incubate over night. Day 2: Transfections Using Mirus TransIT HeLaMonster (check manufacturers protocols if using other reagents)
4. Add the pre-warmed dissociation reagent such as trypsin or TrypLE™ to the side of the flask; use enough reagent to cover the cell layer (approximately 0.5 mL per 10 cm2 ). Gently rock the container to get complete coverage of the cell layer
RNA purification using TRizol--TRIZOL法提取RNA
Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol
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