1423253伯乐填料Bio-rad Chelex 100

Chelex-100法提取微量DNA

Chelex-100法提取微量DNA1. Add 1/50 volume of Proteinase K (10 mg/mL) and incubate the sample at 65°C for 2 hrs to overnight.2. 200 uL 5% Chexlex-100,add 10 uL proteinase K (20 mg/mL),inculate 2 hrs in 37 °C.3. 50ul Chelex液及lfl蛋白酶K水，56％温水浴消化2h后，煮沸8min，迅速放

Coupling Peptides To Resin For Affinity Purification

is light sensitive. Wash with 10 volumes of 0.1 M NaPi pH 7.8= buffer. Resuspend the resin as a 50% slurry in buffer. Add the peptide. If the peptide is soluble directly in buffer, add it as a solid. Many peptides go in better added as a 100

各类western转膜条件

分子量：156.8 WB用膜类型、孔径：NC膜 转膜方式（恒压、恒流）：恒流400mA 转膜时间：90 min 转膜设备：湿转 Bio-Rad   蛋白来源：真核表达 蛋白名称（可保密）：（多种，30个以上） 蛋白分子量：40-200 kD WB用膜类型、孔径：PVDF 转膜方式（恒压、恒流）：恒压100 V（电流在280 mA至350 mA之间） 转膜时间：60 min（M