赛默飞世尔Thermo Fisher FLAT ELECTR

In Vitro and In Vivo Recording of Local Field Potential Oscillations in Mouse Hippocampus

., and Carandini, M. 2009. Local origin of field potentials in visual cortex. Neuron 61:35‐41.    Klausberger, T. and Somogyi, P. 2008. Neuronal diversity and temporal dynamics: The unity

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

    Dissecting microscope;       Acid Tyrode's (e.g. Sigma: T1788);       Sterile plastic Petri dishes (100 x 15 mm);       M2 and KSOM medium in 1 ml syringes;       9'' Pasteur pipettes;       Mouth or Finger controlled pipette. Method: Place

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos

using a platinum wire (80 x 0.3 mm). Bend the wire at the midsection to create a 3-mm segment that is raised 1 mm above the center of the dish and runs parallel to the dish floor (Fig. 1 , bottom electrode). Attach the lengths of wire